Flavonoids in safflower extract reduce cisplatin-induced damage to human follicle dermal papilla cells by inhibiting DNA damage and Rad17/Chk1/Cdc25C signaling

Background: Cisplatin is a chemotherapeutic agent commonly used clinically for the treatment of various human cancers. Patients often reduce use cisplatin due to its side effects, which in turn affects treatment. This study explored mechanism action safflower extract as an adjuvant traditional Chinese medicine chemotherapy. Methods: Primary follicle dermal papilla cells (HFDPCs) were target cisplatin-induced damage hair cells. Western blotting was investigate molecular targets and causing HFDPCs damage. Cell survival cell cycle analyzed by mitochondrial staining reagent WST-1 propidium iodide. Results: could viability without death. increased level phospho-Rad17 activated Chk1/Cdc25C signaling expression Cdc2 protein, thereby arresting G2/M phase. The combination flavonoids effectively inhibit signal transduction Rad17/Chk1/Cdc25 cisplatin-treated population Finally, we also confirmed that caused cisplatin, mainly at reducing DNA cisplatin. Conclusions: Safflower can be chemotherapy normal